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Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling.

Saba-El-Leil MK, Frémin C, Meloche S

Institute for Research in Immunology and Cancer, Université de Montréal, 2950, Chemin de Polytechnique, Montréal, QC, Canada, H3C 3J7.

Mouse embryonic stem (ES) cells have proven to be invaluable research tools for dissecting the role of signaling pathways in embryonic development, adult physiology, and various diseases. ES cells are amenable to genetic manipulation by classical gene targeting via homologous recombination or by genome editing technologies. These cells can be used to generate genetically modified mouse models or to study the signaling circuitry regulating self-renewal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.

Methods Mol. Biol. 2017;1487:243-253.

Pubmed ID: 27924572

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