Cellular and subcellular context determine outputs from signaling biosensors.
Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, Canada.
The use of biosensors either individually or as part of panels has now become a common technique to capturing signaling events in living cells. Such biosensors have become particularly important for probing biased signaling and allostery in G protein-coupled receptor drug screening efforts. However, assumptions about the portability of such biosensors between cell types may lead to misinterpretation of drug effects on specific signaling pathways in a given cellular context. Further, the output of a particular biosensor may be different depending on where it is localized in a cell. Here, we discuss strategies to mitigate these concerns which should feed into future biosensor design and usage.
Methods Cell Biol. 2016;132:319-37.
Pubmed ID: 26928550