Equipment

Microscopes

DeltaVision Elite Inverted Microscope

Instrument equipped with a temperature and CO2 controlled chamber for live-cell imaging over long periods.

  • Software: softWoRx
  • Multidimensional capture in λ, T, Z with capacity for deconvolution
  • Techniques: DIC, epifluorescence
  • Camera : Coolsnap HQ2
  • Objectives : 20x/0.70, 60x/1.42 oil, 100x/1.42 oil
  • Available filters:
    • Live Cell : CFP 470/24, GFP 525/50, YFP 559/38, mCherry 632/60, POL DIC
    • Standard : DAPI 435/4, FITC 525/50, TRITC 594/45, CY5 676/34, POL DIC
    • Alexa set : DAPI 435/48, FITC 525/50, AF594 632/60, CY5 676/34, POL DIC

 

LEICA DMIRB Inverted Microscope
  • Techniques: bright field, dark field, epifluorescence (DAPI, GFP, Cy3)
  • Camera: Retiga EX-i
  • Software: Q-Capture
  • Objectives :
    • 2.5x N-Plan 2.5x / 0.07
    • 5x N-Plan 5x / 0.12 Ph 0
    • 10x HC Pl Fluotar 10x / 0.30, Ph 1
    • 20x N Plan L 20x / 0.4, corr, Ph 1
    • 40x HCX Pl Fluotar L40x / 0.60 corr, Ph 2
LEICA MZ FLIII Fluorescence Stereomicroscope
  • Techniques: bright field, dark field, epifluorescence (GFP)
  • Camera: Canon 5DMkII
  • Software: Canon EOS Utility-remote capture
  • Magnification: (0.8x à 10x) x10
Nikon A1R confocal microscope
  • Nikon Eclipse Ti inverted microscope
  • Acquisitions modes: galvano and resonant (high speed)
  • Laser : 405, 457, 471, 488, 514, 561 and 640 nm
  • Objectives
    • 10x / 0.5 S Fluor
    • 20x / 0.75 S Fluor DIC N2
    • 40x / 0.75 Plan Fluor DIC M N2
    • 60x / 1.4 Plan Apo VC DIC N2
    • 100x / 1.4 Plan Apo DIC, N2
Zeiss Axio-Imager Z 1 Microscope
  • Techniques: bright field, DIC, epifluorescence (DAPI, GFP, Cy3), HBO 100 W lamp
  • Cameras: Zeiss HRM (monochrome) and Canon 5DMKII
  • Software: xio-Vision
  • Objectives:  
    • 10x / 0.45 Plan-Apochromat, DIC I
    • 20x / 0.80 Plan-Apochromat, DIC II
    • 40x / 1.3 Plan-Apochromat, DIC III
    • 63x / 1.4 Plan-Apochromat, DIC III
    • 100x / 1.4 Plan-Apochromat, DIC III

 

Zeiss confocal microscope LSM 700
  • Zeiss Observer Z1 inverted microscope
  • Temperature and CO2 controlled chamber
  • Laser: 405, 488, 555 and 639 nm
  • Techniques: FRAP, FRET and linear unmixing
  • DIC image capture
  • Objectives :
    • 10x / 0.25 N AchroPlan
    • 20x / 0.8 PlanApo DIC
    • 25x / 0.8 LCI PlanApoFluor DIC
    • 40x / 1.3 PlanApo DIC
    • 63x / 1.4 PlanApo DIC
Zeiss confocal microscope LSM 880 NLO, Airyscan
  • Zeiss Observer Z1 inverted microscope
  • Lasers: Multilines Argon, 561 nm and 633 nm
  • 2 Photons
  • Techniques: FRAP, FRET, FLIM (near future)
  • Fast acquisition: up to 13 frames per second
  • Objectives:
    • 10x / 0.25 N AchroPlan, Ph1
    • 20x / 0.8 Plan-Apochromat
    • 40x / 1.0 W Plan-Apochromat, DIC
    • 63x / 1.4 Plan-Apochromat, DIC
Zeiss Spinning Disk confocal microscope
  • « Cell Observer SD »
  • Zeiss Observer Z1 inverted microscope
  • Temperature and CO2 controlled chamber
  • 2 cameras Zeiss AxioCam 506 Mono
  • Laser : 488, 555 and 639 nm
  • Objectives :
    • 10x / 0.3 EC PlanNeoFluor
    • 20x / 0.8 PlanApochromat
    • 40x / 1.3 PlanApochromat
    • 63x / 1.4 PlanApochromat DIC
    • 100x / 1.4 PlanApochromat DIC

Image analysis stations

Three stations equipped with the softwares; NIS-Elements (Nikon), Metamorph (Molecular Devices), softWoRX suite (Applied Precisions), ImageJ (NIH) and IMARIS (BitPlane).

Presentation tools

IRIC’s Bio-Imaging core facility desktop publishing station is equipped with Adobe Photoshop, Illustrator and InDesign as well as premium printing equipment for the production of high quality prints.

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